ABSTRACT
Cancer is a major health problem worldwide and current therapies for cancer are often limited by short-term efficacy due to drug resistance. There has been much interest in the use of naturally occurring compounds with chemo-preventive and chemotherapeutic properties in the treatment of cancers. Rose is one of the most important groups of ornamental plants which their fruits and flowers are used in a wide variety of foods, nutritional products and different traditional medicines. In this study cytotoxic effect of Rosa damascena extract was evaluated on HeLa cell line. HeLa cells were cultured in DMEM medium and incubated with different concentrations of Rosa damascene [R. damascene] extract. Cell viability was quantitated by MTT assay. Rosa decreased cell viability in malignant cells in a concentration and time dependent manner. The IC50 values against HeLa were determined as 2135, 1540 and 305.1 micro g.ml[-1] after 24, 48 and 72h, respectively. It might be concluded that R. damascena could cause cell death in HeLa cells which could be also considered as a promising chemotherapeutic agent in cancer treatment in future
Subject(s)
Uterine Cervical Neoplasms/drug therapy , HeLa Cells/drug effects , Cell Line, Tumor , Medicine, TraditionalABSTRACT
Vaginal candidiasis is one of the most common genital infections, which has many different therapeutic methods. Some Para clinical researches suggest garlic for treatment of candidiasis.The purpose of this study was to compare the effect of garlic extract vaginal douche and clotrimazole vaginal cream in the treatment of women with vaginal candidiasis. This is a randomized, controlled, clinical trial on 100 non-pregnant women who came with the symptomatic vaginal candidiasis. Fresh garlic extract was provided by Pharmacologist, and then the patients whose mycologic culture was positive were randomly allocated into 2 groups of vaginal douche containing 1% garlic extract and vaginal cream containing 1% clotrimazole .All patients were treated daily for 7 days and assessed for clinical and mycological results 1-2 .week after the end of treatment. Data were obtained by interview and observation methods and were analyzed by SPSS software using Chi Square, Fisher exact test student, t-test and paired-samples t-test by the confidence of 95%. Successful response [according to clinical and mycologic results] were reported in 34.7% of Garlic users and 56.9% clotrimazole users. [P=0.026] There was not any significant difference between clinical results after treatment in both groups [51% against 66.7%] but there was a significant difference between mycologic results in both groups. [40.8% against 60.8%] [P=0.046] According to the results, percentage of successful response in users of vaginal garlic extract douche was less than users of vaginal clotrimazole cream but there was no significant statistical difference between these groups according to the clinical result. We suggest more studies about different methods and doses of this drug